Documents from the SIAIP Commissions
Issue 2 - 2024
Multiparametric or multiplex systems in allergy diagnostics
Abstract
Multiparametric or “multiplex” systems allow many and different biological molecules to be tested simultaneously “in vitro”: there are basically two types, one is based on an ISACE112i microarray technology (ISAC) and the other one on an ALEX2 macroarray technology (ALEX). As for any immunodiagnostic assay, the two systems, ISAC and ALEX, are based on specific “designs” concerning the quality of the antigen, substrate, conjugated antibody and isotopic reference system used to calibrate the results. The use of multiplex systems in allergy diagnostics may be appropriate, unnecessary, or even harmful. An unnecessary and harmful use of multiplex systems can determine both risks for the patient such as the prescription of “absurd” extended exclusion diets, a self-diagnosis without a specialist visit, and social-economic risks with unjustified increase of costs by labelling patients as allergic when they actually are not. We have analyzed both these systems, highlighting their virtues, strengths and pitfalls, considering the currently available data in literature and the specific characteristics of each multiplex system. In order to avoid incorrect diagnosis and damage to patients, the choice between the two diagnostic tests has to be tailored to the patient and a prerogative of the specialist with specific knowledge in the field of molecular allergology and able to interpret the results.
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
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Copyright (c) 2024 Italian Journal of Pediatric Allergy and Immunology
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